Gentaur Microscopic stains and reagents for cytology, histology and microbiology

We offer the best prices for these reagents made by our office in Bulgaria

 

contact for prices lieven@gentaur.com

 

  1. Hematoxylin stains

Products Code Size Description

Gill’s hematoxylin I 111601350 500ml For standard haematoxylin-eosin

staining of tissue sections. For nuclei. 111601310 1l

Gill’s hematoxylin II 111601450 500ml For standard haematoxylin-eosin

staining of tissue sections. For nuclei. 111601410 1l

Gill’s hematoxylin III 111601550 500ml For standard haematoxylin-eosin

staining of tissue sections. For nuclei 111601510 1l

Gill I Gill II Gill III

 

 

Mayer’s hematoxylin 111600250 500ml For standard haematoxylin-eosin

staining of tissue sections. For nuclei 111600210 1l

Harris’ hematoxylin 111600450 500ml For standard haematoxylin-eosin

staining of tissue sections. For nuclei 111600410 1l

Weigert’s Hematoxylin 130880100 100 test For trichrome stains

130880250 250ml

130880500 500ml

130880010 1l

Harris Mayer Weigert

 

 

  1. Eosin

Products Code Size Description

Eosin Y 1% aqueous sol. 111000050 50ml Standard haematoxylin-eosin staining

111000300 300ml for cytoplasm

111000250 500ml

111000210 1l

Eosin Y 5% aqueous sol. 111000050.5 50ml Standard haematoxylin-eosin staining

111000250.5 500ml for cytoplasm

111000210.5 1l

Eosin Y 1% aqueous sol. Eosin Y 5% aqueous sol.

 

 

 

Eosin Y 0.5% alcoholic sol. 111000305 50ml Standard haematoxylin-eosin staining

111000325 250ml for cytoplasm

111000350 500ml

111000310 1l

Eosin-Phloxin B alcoholic sol. 111002005 50ml Standard haematoxylin-eosin staining

111002025 250ml for cytoplasm

111002050 500ml

111002010 1l

Eosin Y 0,5% alcoholic sol. Eosin-Phloxin B alcoholic sol.

 

 

  1. Staining solutions for histology and cytology

Products Code Size Description

Alcian blue pH1 162802050 100test For demonstration of acid mucins in

tissue sections 162802250 250ml

162802500 500ml

162802100 1l

Alcian blue pH2.5 161802050 100test For demonstration of carboxylated and

some weakly sulfated mucins in tissue

sections

161802250 250ml

161802500 500ml

161802100 1l

Alcian blue pH1 – P.A.S. 111200100.1 100test For neutral and acid mucins in tissue

sections 111200101.1 100ml

111200250.1 250ml

111200500.1 500ml

111200010.1 1l

Alcian blue pH2.5 – P.A.S. 111200100 100test For neutral and acid mucins in tissue

sections 111200101 100ml

111200250 250ml

111200500 500ml

111200010 1l

 

Alcian Yellow – Toluidine Blue 111300100 100test For screening patient specimens for

Helicobacter pilory 111300250 250ml

Azan Trichrome 121111100 100test Method for connective tissue, especially

to show muscle fibers, collagen,

reticulum, kidney glomerular stroma in

tissue sections

121111250 250 ml

121111010 1l

Brilliant Cresyl Blue for

reticulocytes

111400050 100test Blood smears examination for

reticulocytes 111400125 125ml

111400250 250ml

111400500 500ml

111401000 1l

Bennhold’s Congo red for amyloid 131003050 100test Stain for amyloid deposits in tissues

131003250 250ml

131003500 500ml

131003100 1l

Highman’s Congo red for amyloid 131103205 100test Stain for amyloid deposits in tissues

131103250 250ml

131103500 500ml

131103100 1l

CytoMax – fast Papanicolau for

cyto PAP smear

221222050 100test For cyto PAP and other smears

(cytological specimens) 221222100 100ml

221222250 250ml

221222500 500ml

 

Giemsa stain 111120100 100test Giemsa concentrate solution

111120250 250ml

111120500 500ml

111120010 1l

Gomori silver impregnation 114080100 100test Method to show argyrophilic reticular

fibers in connective tissue

Gram for microbiology 100802100 100test Differentiation between Gram positive

and Gram negative bacteria in different

specimens

100802125 100ml

100802250 250ml

100802500 500ml

100802010 1l

Grocott’s methenamine silver

nitrate method for fungi

143823100 100test To identify fungi in tissue section

143823010 100ml

Haematocolor (hematocolor) 111316050 100test Blood smear examinations

111316100 100ml

111316250 250ml

111316500 500ml

Leishman stain 113273100 100test Blood smear examinations

113273125 125ml

113273250 250ml

113273500 500ml

 

 

Leishman-Wright stain 113273100W 100test Blood smear examinations

113273125W 125ml

113273250W 250ml

113273500W 500ml

Mallory trichrome 120802100 100test Staining solution for connective tissue

Masson trichrome Goldner 110802100.1 100test Staining solution for connective

tissue – collagen and muscle fiber

Masson trichrome Standard 110802100 100test Staining solution for connective

tissue – collagen and muscle fiber

May Grunwald 111200100 100test Fixative for MGG blood smear

examinations 111200250 250ml

111200500 500ml

111200010 1l

May Grunwald – Giemsa 118080100 100test Blood smear examination

118080010 1l

Methenamine Silver Impregnation

(P.A.S.M.)

114382100 100test Kit for histology (lamina basalae,

argyrophil cells etc.).

Methylene Blue Loffler 112000100 100test Alkaline stain for cell nuclei in smears

from different origin and pathogens 112000250 250ml

112000500 500ml

112000010 1l

 

 

New Methylene Blue for

reticulocytes

 

1114003050 100test Blood smear examination for

reticulocytes 1114003125 125ml

1114003250 250ml

Oil Red O for lipids in frozen

sections

122092100 100test Lipid staining in frozen sections

122092125 125ml

122092250 250ml

Orcein 155802100 100test To demonstrate elastic fibers in tissue

sections, recomended for vascular

pathology

155802250 250ml

Papanicolau – OG6 112220250 250ml Stain solution I for cyto PAP smears

112220500 500ml

112220010 1l

Papanicolau – EA65 112230250 250ml Stain solution II for cyto PAP smears

112230500 500ml

112230010 1l

Papanicolau – EA50 112230250.1 250ml Stain solution II for cyto PAP smears

112230500.1 500ml

11223010.1 1l

Periodic Acid Schiff (P.A.S.) 113080100 100test Examination of mucosubstances

(mucopolysaccharides), lamina

basalae, glycogen deposits in liver etc.

in tissue sections

113080101 100ml

113080250 250ml

113080500 500ml

 

 

13

Rapihem 111317050 100test Blood smears, other cytological

specimens and fine needle aspirations

examination

111317250 250ml

111317500 500ml

111317100 1l

Sudan III Propylene Glycol 112700050 100test Triglyceride staining in frozen sections

112700250 250ml

112700500 500ml

112700100 1l

Sudan III alcohol solution 112700050A 100test Staining of triglycerides in frozen tissue

sections. 112700250A 250ml

112700500A 500ml

112700100A 1l

Toluidine Blue stain for hystology 123001050 100test To demonstrate metachromatic

substances and mast cells in tissue

sections

123001250 250ml

123001500 500ml

123001100 1l

Van Gieson Trichrome stain 130802100 100test Staining for connective tissue

130802125 125ml

130802250 250ml

130802500 500ml

Weigert for elastic fibers 124001100 100test To demonstrate elastic fibers in tissue

sections 124001125 125ml

 

 

14

Wright’s Stain for cytology 113273100WP 100test Fine needle aspirations and other

cytological specimens examination 113273125WP 125ml

113273250WP 250ml

113273500WP 500ml

Ziehl Neelsen cold 116450050 100test Microscopic examination of

Mycobacteria 116450250 250ml

116450500 500ml

 

15

 

 

ALCIAN BLUE pH 1

number of tests 100

procedure time 50 min. (20 min. microwave oven modification)

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Weakly and strongly sulfated acid mucins Blue

Nuclei Red to pink

Other tissue elements Pale pink

Alcian blue is used for the demonstration of acid mucins, which can be produced by several types of epithelial and connective tissue tumors. Acid mucins also

play role in collagen diseases and early stages of atherosclerosis.

Reagents:

A – Alcian blue staining solution рН 1 B – Nuclear Fast Red staining solution

Alcian blue pH1 reaction – intestine

16

 

 

ALCIAN BLUE pH 2,5

number of tests 100

procedure time ~50 min. (20 min. microwave oven modification)

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, in glass dark

bottle, tightly closed

Carboxylated and some weakly sulfated

acid mucins

Blue

Nuclei Red to pink

Other tissue elements Pale pink

Alcian blue is used for the demonstration of acid mucins, which can be produced by several types of epithelial and connective tissue tumors. Acid mucins also

play role in collagen diseases and early stages of atherosclerosis.

Reagents:

A – Alcian blue staining solution рН 2,5 B – Nuclear Fast Red staining solution

Alcian blue pH2,5 reaction – intestine

17

 

 

ALCIAN BLUE pH 1 – P.A.S.

number of tests 100

procedure time ~50 min. (20 min. microwave oven modification)

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Weakly and strongly sulfated acid mucins Blue

Neutral mucins Magenta

Mixtures of acid and neutral mucins Bluish to reddish purple

Nuclei (if contra-stained) Blue to black

Alcian blue and PAS reactions can be combined to demonstrate both neutral

and acid mucins in tissue sections.

Reagents:

A – Alcian blue staining solution рН 1

C – Schiff Reagent

B – Periodic acid solution

Alcian blue pH1-PAS reaction – intestine

18

 

 

ALCIAN BLUE pH 2,5 – P.A.S.

number of tests 100

procedure time ~50 min. (20 min. microwave oven modification)

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, in glass dark

bottle, tightly closed

Carboxylated and some faintly acid

sulfomucins

Blue

Neutral mucins Magenta

Mixtures of acid and neutral mucins Bluish to reddish purple

Nuclei (if contra-stained) Blue to black

Alcian blue and PAS reactions can be combined to demonstrate both neutral

and acid mucins in tissue sections.

Reagents:

A – Alcian blue staining solution рН 2,5

C – Schiff Reagent

B – Periodic acid solution

Alcian blue pH2,5 reaction – intestine

19

 

 

ALCIAN YELLOW -TOLUIDINE BLUE

number of tests 100

procedure time ~25 min.

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Helicobacter pilory Dark blue

Mucins Yellow

Background Blue scale

Staining solution for screening patient specimens for Helicobacter pilory –

spiral shaped bacterium implicated in gastric inflammation, peptic ulcers and gastric

cancer.

Reagents:

А – Periodic acid

С – Alcian yellow staining solution

Е – Sodium hydroxide solution

В – Sodium bisulfite solution

D – Toluidine blue staining solution

Alcian yellow-Toluidine blue reaction – intestine

20

 

 

AZAN TRICHROME

number of tests 100

procedure time ~100 min.

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Collagen, reticulum, and kidney glomerular stroma Deep blue

Muscle Orange

Erythrocytes Red

Recommended method for connective tissue, especially to show muscle

fibers, collagen, reticulum, kidney glomerular stroma in tissue sections.

Reagents:

А – Azocarmine solution

C – Differentiation solution

E – Staining solution

В – Aniline solution

D – Phosphomolybdic acid solution

Azan trichrome staining: A) kidney; B)uterus

A B

21

 

 

BRILLIANT CRESYL BLUE

number of tests 100

procedure time ~35 min.

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Reticulocytes Greenish to blue with dark blue ribonucleoprotein granules inside

Erythrocytes Greenish to blue

Blood smears examination for reticulocytes. Brilliant cresyl blue is more used

in hematology laboratories than in histology laboratories. It is invaluable for the staining

of reticulocytes whereas its use in histology is limited to vital staining.

Reagents:

Brilliant Cresyl Blue physiological solution

Brilliant Cresyl Blue – blood smear

22

 

 

BENNHOLD’S CONGO RED

number of tests 100

procedure time ~90 min.

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Amyloid Pink to red (apple-green birefringence

with polarized light)

Nuclei Blue

Stain for amyloid deposits in tissues. Appropriate for tissue sections from

5-12μm. Amyloid deposits are formed in tissues spontaneously or in association with

a wide range of diseases. In cases of suspected amyloidosis, thick sections at 12 μm

may be beneficial in demonstrating minute deposits of amyloid.

Reagents:

A – Congo red staining solution according

Benhold

C – Mayer’s haematoxylin solution

B – Sodium hydroxide solution

Bennhold’s congo red for amyloid – kidney

23

 

 

HIGHMAN’S CONGO RED

number of tests 100

procedure time ~20 min.

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Amyloid Pink to red (apple-green birefringence

with polarized light)

Nuclei Blue if contra-stained

Stain for amyloid deposits in tissues. Suitable for paraffin sections fixed in

10% formalin. Amyloid deposits are formed in tissues spontaneously or in association

with a wide range of diseases. In cases of suspected amyloidosis, thick sections at 12

μm may be beneficial in demonstrating minute deposits of amyloid.

Reagents:

A- Congo red staining solution according

Highman

B- Potassium hydroxide solution

Highman’s congo red for amyloid – kindey

24

 

 

CYTOMAX

number of tests 100

procedure time 3 min.

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Nuclei Violet-blue to black

Cytoplasm (eosinophilic) Pink

Cytoplasm (basophilic) Turquoise to blue

Cytoplasm (metaplasia cells) With both blue and pink

Eritrocytes Red

Microorganisms Blue-violet

Stain solution for cyto PAP and other smears (cytological specimens).

Reagents:

А – Harris`s haematoxylin solution

C – Modified polychromic EA65 solution

B – 2-Propanol Absolute

D – 80% 2-Propanol

Cytomax – cyto PAP smear

25

 

 

GIEMSA STAIN

number of tests 100

procedure time ~40 min.

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Lymphocytes Light blue cytoplasm

Monocytes U shaped nucleus

Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (with dark violet to black granules)

Platelets Violet

Eritrocytes Light red

Blood parasites Red

Blood smears examination.

Reagents:

Giemsa concentrate solution

Giemsa stain – blood smear

26

 

 

GOMORI SILVER IMPREGNATION

Gomori silver impregnation – kidney

number of tests 100

procedure time ~30 min.

product validity 1 year (under recomended conditions)

storage conditions 15-25°C, protected from fire and sunlight, in glass dark bottle,

tightly closed

Reticular fibers and nervous cells Black

Connective tissue Tobacco brown

Collagen Gold yellow

Recommended method to show argyrophilic reticular fibers in connective tissue, especially to differentiate collagen fibers from connectivum.

Reagents:

А – Potassium permanganate solution

С – Oxalic acid solution

E – Ammoniacal silver solution

G – Sodium hyposulphite solution

В – Acid activating buffer

D – Ferric ammonium sulfate solution

F – Neutral formalin solution

27

 

 

GRAM

number of tests 100

procedure time 5 min.

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Gram positive microorganisms Blue

Gram negative microorganisms Red

Differentiation between Gram positive and Gram negative bacteria in different

specimens.

Reagents:

A – Crystal Violet solution

C – Decolorising solution

B – Lugol’s solution for Gram

D – Safranin O alcoholic solution

Gram staining – bacterial speciments

28

 

 

GROCOTT for fungi

Grocott’s method for fungi – gastric tissue

number of tests 100

procedure time ~100 min. (~25 min. – microwave oven modification)

product validity 1,5 years (under recomended conditions)

storage conditions 4°C, protected from fire and sunlight, tightly closed

Fungi Black

Mucin Grey

Background Green

To identify fungi in tissue sections. The mucopolysaccharide components of

the fungal cell wall are oxidized to release aldehyde groups. The aldehyde groups then

react with the silver nitrate, reducing it to a metallic silver, rendering them visible.

Reagents:

А – Chromic acid solution

С – Methenamine solution

E – Borax water solution

G – Sodium thiosulfate solution

В – Sodium hydrogensulfite solution

D – Silver nitrate solution

F – Gold chloride water solution

H – Light Green solution

29

 

 

HAEMATOCOLOR (HEMATOCOLOR)

number of tests 100

procedure time 1 min.

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Blood smears examination.

Reagents:

A – Methanol absolute

C – Haematocolor Solution II

B – Haematocolor Solution I

D – Haematocolor Phosphate buffer

Lymphocytes Bluish

Monocytes U shaped nucleus

Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (dark stained due to the large histamine

granules)

Platelets Purple

Eritrocytes Purple-pinkish

Haematocolor – blood smear

30

 

 

LEISHMAN STAIN

number of tests 100

procedure time 7 min.

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Blood smears examination.

B – Buffered water

Reagents:

A – Leishman staining solution

Leishman stain – blood smear

Lymphocytes Bluish

Monocytes U shaped nucleus

Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (dark stained due to the large histamine

granules)

Platelets Pink-violet

Eritrocytes Pink

31

 

 

LEISHMAN-WRIGHT STAIN

number of tests 100

procedure time 7 min.

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Blood smears examination.

B – Buffered water

Reagents:

A – Leishman-Wright staining solution

Leishman-Wright stain – blood smear

Lymphocytes Bluish

Monocytes U shaped nucleus

Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (dark stained due to the large histamine

granules)

Platelets Pink-violet

Eritrocytes Pink

32

 

 

MALLORY TRICHROME

number of tests 100

procedure time ~20 min.

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Nuclei Red

Erythrocytes Orange red

Muscle Red

Collagen Blue

Staining solution for connective tissue.

Reagents:

А – Acid fuchsin staining solution

C – Mallory’s staining solution

B – Phosphomolybdic acid solution

Mallory trichrome – placenta

33

 

 

MASSON TRICHROME STANDARD

number of tests 100

procedure time ~40 min (+/- pre-fixation time).

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Nuclei Black

Muscle fibers, cytoplasm, erythrocytes Red

Collagen, mucus Blue

Staining solution for histology. Collagen and muscle fiber stain differentially

when treated sequentially with Acid Fuchsin, PTA/PMA and Aniline Blue.

Reagents:

A- Weigert’s Haematoxylin solution A

C- Acid fuchsin staining solution

E- Phosphotungstic acid solution

G- Acetic acid solution

B- Weigert’s Haematoxylin solution B

D- Phosphomolybdic acid solution

F- Masson’s aniline blue solution

Masson trichrome standard – uterus

34

 

 

MASSON TRICHROME GOLDNER

Masson trichrome Goldner – uterus

number of tests 100

procedure time ~40 min. (+/- pre-fixation time).

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Staining solution for histology. Collagen and muscle fiber stain differentially

when treated sequentially with Acid Fuchsin, PTA and Light green.

Reagents:

A- Weigert’s Haematoxylin solution A

C- Acid fuchsin staining solution

E- Light green staining solution

Nuclei Black

Muscle fibers, cytoplasm, erythrocytes Red

Collagen

B- Weigert’s Haematoxylin solution B

D- Phosphotungstic acid solution

Greenish

35

 

 

MAY GRUNWALD STAIN

number of tests 100

procedure time ~2 min. (+ Giemsa co-staining)

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Supplementary stain for May Grunwald – Giemsa blood smear examination.

Reagents:

May – Grunwald Stain solution

White blood cells Faintly shaped

Erythrocytes Pinkish-red

May Grunwald stain – blood smear

May Grunwald stain only May Grunwald – Giemsa

36

 

 

MAY GRUNWALD – GIEMSA

number of tests 100

procedure time ~10-35 min.

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Blood smear examination and cytodiagnosis.

Reagents:

A – Methanol absolute

C – Giemsa staining solution

Lymphocytes Light blue cytoplasm

Monocytes U shaped nucleus

Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (with dark violet to black granules)

Platelets Violet

Eritrocytes Reddish brown

Blood parasites Red

B – May-Grunwald staining solution

D – Sorenson buffer pH7

May Grunwald Giemsa – blood smear

37

 

 

METHENAMINE SILVER IMPREGNATION – P.A.S.M.

P.A.S.M. – kidney

number of tests 100

procedure time ~2 h.

product validity 1 year (under recomended conditions)

storage conditions 4 °C, in dark glass bottle, protected from fire and sunlight,

tightly closed

Kit for histology (lamina basalae, argyrophil cells etc.).

Reagents:

А – Periodic acid solution

C – Hexamethylene tetraamine solution

E – Gold chloride solution

Lamina basalae Black

Nuclei and background Pinkish-red and green if contra-stained

Positive control Skin, kydney

В – Silver nitrate solution

D – Sodium tetraborate solution

F – Fixative

38

 

 

METHYLENE BLUE LOFFLER

Methylene Blue Loffler: A) mouth epithel; B)blood smear

number of tests 100

procedure time ~5 min.

product validity 2 years (under recomended conditions)

storage conditions 15-25 °C, protected from fire and sunlight, tightly closed

Alkaline stain for cell nuclei in smears from different origin. Pathogens (bacteria) examination.

Reagents:

Methylene Blue solution according Loffler

Bacteria Blue

Leucocytes nuclei Blue

Epithelial cells cytoplasm Slightly blue

Epithelial cells nuclei Blue

A B

39

 

 

NEW METHYLENE BLUE

New Methylene Blue – blood smear

number of tests 100

procedure time ~20 min.

product validity 2 years (under recomended conditions)

storage conditions 15-25 °C, protected from fire and sunlight, tightly closed

Blood smears examination for reticulocytes. Reticulocytes are juvenile red

cells, which contain remnants of basophilic ribonucleoproteins. This material reacts

with the dye to form a bluish black precipitate of granules or filaments.

Reagents:

New Methylene Blue water, isotonic solution, tilled with anticoagulant

Reticulocytes Greenish to blue with dark blue

ribonucleoprotein granules inside

Erythrocytes Greenish to blue

40

 

 

OIL RED O

Oil red O – omentum cryostate section

number of tests 100

procedure time ~40 min.

product validity 2 years (under recomended conditions)

storage conditions 15-25 °C, protected from fire and sunlight, tightly closed

Lipid staining in frozen sections.

Reagents:

A – Propylene glycol absolute

C – Propylene glycol 85% water solution

Nuclei Blue to black

Lipids

B – Staining solution Oil red O

D – Mayer’s haematoxylin solution

Red

41

 

 

ORCEIN for elastic fibers

number of tests 100

procedure time ~30 min

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Elastic fibers Purplish red-brown

Background Almost colourless

To demonstrate elastic fibers in tissue sections; recommended for vascular

pathology.

Reagents:

А – Potassium permanganate solution

С – Oxalic acid solution

Е – Orcein solution according Shikata

В – Acid activation buffer

D – Incubation media

F – Differentiation solution

Orcein for elastic fibers: A) blood vessels; B) kidney

A B

42

 

 

PAPANICOLAU

number of tests 100

procedure time ~25 min

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Stain solution for cyto PAP smears.

N.B! EA65 ( Biotech Ltd.) is an alternative for EA50 stain, providing more intensive cytoplasm staining!

Reagents:

А – Harris`s haematoxylin solution

C – EA50/65 polychromic stain solution

В – Orange G-6 stain solution

Papanicolau for cyto PAP smears: A) EA 50; B)EA 65

Nuclei Orange-brown to bluish-black

Cytoplasm (keratinized) Orange-pink

Cytoplasm (basophilic) Blue-green (EA50) to turquoise (EA65)

Cytoplasm (metaplasia cells) With both blue and pink

Cytoplasm (eosinophilic) Pink

Eritrocytes Red

Microorganisms Blue-violet to blue-black

A B

43

 

 

PERIODIC ACID SCHIFF (P.A.S.)

PAS reaction – gastric tissue (stomach)

number of tests 100

procedure time ~35 min.

product validity 2 years (under recomended conditions)

storage conditions 15-25 °C,in dark glass bottle, protected from fire and sunlight,

tightly closed

Examination of mucosubstances (mucopolysaccharides), lamina basalae,

glycogen deposits in liver etc. in tissue sections.

Reagents:

A – Periodic acid solution

C – Mayer’s haematoxylin solutionn

Glycogen, fungi, neutral mucosubstances, and some lamina basalae Magenta

Nuclei

B – Schiff Reagent

Blue

44

 

 

RAPIHEM

number of tests 100

procedure time ~ 40 sec.

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Cells Clear shaped morphology (nucleus,

cytoplasm), due to specimen origin! Enabling interpretation of their methabolitic

condition

Blood smears, other cytological specimens and fine needle aspirations examination.

Recommendable for fast blood smears examination!

Reagents:

A – Rapihem fixative

С – Rapihem solution II

В – Rapihem solution I

D – Rapihem washing solution –

concentrate

A

Rapihem : A) Fine needle aspiration; B) Blood smear

B

45

 

 

SUDAN III – alcohol solution

Sudan III alcohol solution – omentum cryostate section

number of tests 100

procedure time ~35 min.

product validity 2 years (under recomended conditions)

storage conditions 15-25 °C, protected from fire and sunlight, tightly closed

Staining of triglycerides in frozen tissue sections.

Reagents:

A – 50% Ethanol

C – Mayer’s haematoxylin solution

Nuclei Blue to black

Lipids

B – Staining solution Sudan III (alcoholic

Orange

solution)

46

 

 

SUDAN III – Propylene Glycol

Sudan III Propylene Glycol – omentum cryostate section

number of tests 100

procedure time ~25 min.

product validity 2 years (under recomended conditions)

storage conditions 15-25 °C, protected from fire and sunlight, tightly closed

Triglycerides staining in frozen sections.

Reagents:

A – Staining solution Sudan III

C – 50% Propylene glycol water solution

Nuclei Blue to black

Lipids

B – 85% Propylene glycol water solution

D – Mayer’s haematoxylin solution

Orange

47

 

 

TOLUIDINE BLUE

Toluidine Blue staining: A) skin; B) uterus

number of tests 100

procedure time ~6 min.

product validity 2 years (under recomended conditions)

storage conditions 15-25 °C, protected from fire and sunlight, tightly closed

  1. To demonstrate mucopolysaccharides and other metachromatic substances

in tissue sections. 2. To demonstrate mast cells (with histamine, heparin metachromatic

granules) in tissue sections.

Reagents:

Toluidine blue solution

Tissue structure Visible like in standard Haematoxylin/

Eosin histology staining, but in blue scale.

Mast cells Violet metachromatic staining

Positive control Skin

A B

48

 

 

VAN GIESON TRICHROME

Van Gieson trichrome staining: A) skin; B) placenta

number of tests 100

procedure time ~35 min.

product validity 2 years (under recomended conditions)

storage conditions 15-25 °C, protected from fire and sunlight, tightly closed

Staining of connective tissue.

Reagents:

A – Weigert’s Haematoxylin solution B

C – Van Gieson’s picrofuchsin

Nuclei Black

Collagen fibers Red

Muscle, cornified epithelium, erythrocytes

B – Weigert’s Haematoxylin solution B

Yellow

A B

49

 

 

WEIGERT for elastic fibers

Weigert for elastic fibers – kidney

number of tests 100

procedure time ~50 min.

product validity 2 years (under recomended conditions)

storage conditions 15-25 °C, protected from fire and sunlight, tightly closed

To demonstrate elastic fibers in tissue sections; recommended for vascular

pathology.

Reagents:

А – Periodic acid solution

С – Weigert’s resorcin fuchsin soution

Е – Carmalum according to Mayer

solution

Elastic fibers Purplish red-brown

Nuclei

В – Alcoholic reagent for incubation box

D – Acid differentiation buffer

Red

50

 

 

WRIGHT’S STAIN for cytology

number of tests 100

procedure time ~2 min

product validity 2 years (under recomended conditions)

storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed

Fine needle aspirations and other cytological specimens examination

Reagents:

A – Methanol absolute

С – Concentrated Phosphate buffer

рН 6,8

В – Wrght’s staining solution

Wright’s Stain for cytology – fine needle aspiration

Cells Clear shaped morphology (nucleus,

cytoplasm), due to specimen origin! Enabling interpretation of their methabolitic

condition

Erythocytes Pinkish-green

51

 

 

COLD ZIEHL-NEELSEN

Ziehl-Neelsen stain – sputum

number of tests 100

procedure time ~45 min.

product validity 2 years (under recomended conditions)

storage conditions 15-25 °C, protected from fire and sunlight, tightly closed

Microscopic examination of Mycobacteria.

Higher concentration of basic fuchsin and phenol in Kinyoun’s carbol fuchsin solution

helps penetration of Mycobacteria’s cell wall and removes the need for pre-heating

the slides.

Reagents:

А – Kinyoun’s carbol fuchsin solution

С – Methylene blue (water) staining solution

Tubercle bacilli Bright red

Background

В – Acid alcohol solution

Light blue

 

 

52

  1. Reagents I

Method Reagent Stability

ALCIAN BLUE pH 1- PAS A – Alcian blue staining solution рН 1 2

B – Periodic acid solution 2

C – Schiff Reagent 2

ALCIAN BLUE pH 2.5– PAS A – Alcian blue staining solution рН 2,5 2

B – Periodic acid solution 2

C – Schiff Reagent 2

ALCIAN BLUE pH 2.5 A – Alcian blue staining solution рН 2,5 2

B – Nuclear Fast Red staining solution 2

ALCIAN BLUE pH 1 A – Alcian blue staining solution рН 1 2

B – Nuclear Fast Red staining solution 2

ALCIAN YELLOW –

TOLUIDINE BLUE

А – Periodic acid solution 2

В – Sodium bisulfite solution 2

С – Alcian yellow staining solution 2

D – Toluidine blue staining solution 2

Е – Sodium hydroxide solution 2

AZAN TRICHROMЕ А – Azocarmine solution 2

В – Aniline solution 2

C – Differentiation solution 2

 

 

53

D – Phosphomolybdic acid solution 2

E – Staining solution 2

BRILLIANT CRESYL BLUE Brilliant Cresyl Blue physiological solution 2

BENNHOLD’S CONGO RED A – Congo red staining solution according

Benhold

2

B – Sodium hydroxide solution 2

C – Mayer’s haematoxylin 2

HIGHMAN’ S CONGO RED A- Congo red staining solution according

Highman

2

B- Potassium hydroxide solution 2

CYTOMAX А – Harris`s haematoxylin solution 2

B – 2-Propanol Absolute 2

C – Modified polychromic EA65 solution 2

D – 80% 2-Propanol 2

EOSIN –

ACQUEOUS SOLUTION 1%

Eosin Y 1% water solution 2

EOSIN –

ACQUEOUS SOLUTION 5%

Eosin Y 5% water solution 2

EOSIN –

ALCOHOLIC SOLUTION 0.5%

Eosin Y 0.5% alcoholic solution 2

EOSIN-PHLOXINE

ALCOHOLIC SOLUTION

А – Mayer’s haematoxylin 2

В – Scott’ s solution (water) 2

С – Eosin-Phloxine staining solution 2

GIEMSA STAIN Giemsa concentrate solution 2

GILL’S I HAEMATOXYLIN Gill’s 1 Haematoxylin solution 2

 

 

54

GILL’S II HAEMATOXYLIN Gill’s 2 Haematoxylin solution 2

GILL’S III HAEMATOXYLIN Gill’s 3 Haematoxylin solution 2

GOMORI SILVER

IMPREGNATION

А – Potassium permanganate solution 2

В – Acid activating buffer 2

С – Oxalic acid solution 2

D – Ferric ammonium sulfate solution (alum) 2

E – Ammoniacal silver solution 1

F – Neutral formalin solution 2

G – Sodium hyposulphite solution 2

GRAM STAIN A – Crystal Violet solution 2

B – Lugol’s solution for Gram 2

C – Decolorising solution 2

D – Safranin O alcoholic solution 2

GROCOTT’S А – Chromic acid solution 2

В – Sodium hydrogensulfite solution 2

С – Methenamine solution 2

D – Silver nitrate solution 2

E – Borax water solution 2

F – Gold chloride water solution 2

G – Sodium thiosulfate solution 2

H – Light Green solution 2

HAEMATOCOLOR A – Methanol absolute 2

B – Haematocolor solution I 2

 

 

55

C – Haematocolor solution II

2

D – Haematocolor Phosphate buffer

2

HARRIS’S HAEMATOXYLIN Harris’s haematoxylin solution

2

LEISHMAN STAIN A – Leishman Staining solution

2

B – Buffered water

2

LEISHMAN- WRIGHT STAIN A – Leishman-Wright staining solution

2

B – Buffered water

2

MALLORY TRICHROMЕ А – Acid fuchsin staining solution

2

B – Phosphomolybdic acid solution

2

C – Mallory’s staining solution

2

MASSON TRICHROME

GOLDNER

A- Weigert’s Haematoxylin solution A

2

B- Weigert’s Haematoxylin solution B

2

C- Acid fuchsin staining solution

2

D- Phosphotungstic acid solution

2

E- Light green staining solution

2

MASSON TRICHROME

STANDARD

A- Weigert’s Haematoxylin solution A

2

B- Weigert’s Haematoxylin solution B

2

C- Acid fuchsin staining solution

2

D- Phosphomolybdic acid solution

2

E- Phosphotungstic acid solution

2

F- Masson’s aniline blue solution

2

G- Acetic acid solution

2

MAY GRÜNWALD – GIEMSA A – Methanol absolute

2

 

 

56

B – May-Grunwald staining solution 2

C – Giemsa staining solution 2

D – Sorenson (Sorensen) buffer pH7 2

MAYER’S HAEMATOXYLIN Мayer’s Haematoxylin solution 2

MAY – GRUNWALD STAIN May – Grunwald Stain solution 2

METHENAMINE

SILVER IMPREGNATION –

P.A.S.M

А – Periodic acid solution 2

В – Silver nitrate solution 1

C – Hexamethylene tetraamine solution 1

D – Sodium tetraborate solution 2

E – Gold chloride solution 1

F – Fixative 2

METHYLENE BLUE LӦFFLER Methylene Blue solution according Loffler

NEW METHYLENE BLUE New Methylene Blue water, isotonic solution,

tilled with anticoagulant

OIL RED O A – Propylene glycol absolute

B – Staining solution Oil red O

C – Propylene glycol 85% water solution

D – Mayer’s haematoxylin solution

ORCEIN А – Potassium permanganate solution

В – Acid activation buffer

С – Oxalic acid solution

D – Incubation media

Е – Orcein solution according Shikata

F – Differentiation solution

 

 

57

PAPANICOLAU А – Harris`s haematoxylin solution

В – Orange G-6 stain solution

C – EA50 polychromic stain solution

C – EA65 polychromic stain solution

PERIODOC ACID SCHIFF

(PAS)

A – Periodic acid solution

B – Schiff Reagent

C – Mayer’s haematoxylin solution

RAPIHEM A – Rapihem fixative

В – Rapihem solution I

С – Rapihem solution II

D – Rapihem washing solution – concentrate

SUDAN III

PROPYLENE GLYCOL

A – Staining solution Sudan III

B – 85% Propylene glycol water solution

C – 50% Propylene glycol water solution

D – Mayer’s haematoxylin solution

SUDAN III

ALCOHOL SOLUTION

A – 50% Ethanol

B – Staining solution Sudan III (alcoholic

solution)

C – Mayer’s haematoxylin solution

TOLUIDINE BLUE STAIN Toluidine blue solution

VAN GIESON TRICHROME

STAIN

A – Weigert’s Haematoxylin solution A

B – Weigert’s Haematoxylin solution B

C – Van Gieson’s picrofuchsin.

WEIGERT for elastic fibers А – Periodic acid solution

 

 

58

В – Alcoholic reagent for incubation box

С – Weigert’s resorcin fuchsin

D – Acid differentiation buffer

Е – Carmalum according to Mayer

WEIGERT’S HAEMATOXYLIN A – Weigert’s Haematoxylin solution A

B – Weigert’s Haematoxylin solution B

WRIGHT’S STAIN for cytology A – Methanol absolute

В – Wrght’s staining solution

С – Concentrated Phosphate buffer рН 6,8

COLD ZIEHL-NEELSEN A – Kinyoun’s carbol-fuchsin solution

B – Acetic alcohol solution

C – Methylene Blue solution

 

 

59

  1. Reagents II

Products Code Size Description

Acid ethanol Shikata 155802125E 125ml Differentiating agent

155802250E 250ml

155802500E 500ml

Acid ethanol Weigert 153812125C 125ml Differentiating agent

153812250C 250ml

153812500C 500ml

153812100C 1l

Hidrocloric Acid (1M) 1090570125 125ml Differenting agent

1090570250 250ml

1090570500 500ml

1090570100 1l

Kovach’s indole reagent 119002125 125ml For identification of indole

119002250 250ml

119002500 500ml

119002100 1l

Orcein Acetic 1558011125 125ml For chromosomal staining

 

 

60

Oxalic Acid 1099650125 125ml Decolorant

1099650250 250ml

1099650500 500ml

1099650100 1l

Phosphate buffer pH 6.8 113273125 125ml

113273250 250ml

113273500 500ml

113273100 1l

Phosphate buffer pH 7.2 111316125D 125ml

111316250D 250ml

111316500D 500ml

111316100D 1l

Picric Acid aqueous sol. 1.2% 110901125 125ml For fixatives mixtures

110901250 250ml

110901500 500ml

110901100 1l

Picric Acid alcoholic sol. 110902125 125ml For erythrocytes in trichrome staining

110902250 250ml

110902500 500ml

110902100 1l

Picrofuchsin 1159270125 125ml For cytoplasm, muscle and collagen

 

 

61

Potassium Methabisulfite 0.5% 105057125 125ml Differentiating agent

105057250 250ml

105057500 500ml

105057100 1l

Sodium Thiosulphate 5% 109950125 125ml Decolorant after treatment with lugol

solution 109950250 250ml

109950500 500ml

109950100 1l

Sulphuric Acid 0.5% 109981125 125ml For oxidation with permanganate

109981250 250ml

109981500 500ml

 

 

62

Product Supplementary Code Description

DEK – HL DEK-HL decalcifying solution 111300910 For fibrillar and

lamellar bone

structure Acid solution for DEK-HL decalcifying solution 111300910.1

Ammonium solution for DEK-HL decalcifying solution 111300910.2

DEK – HN DEK-HN decalcifying solution 111300510 For bone tissue

Sodium solution for DEK-HN decalcifying solution 111300510.1

DEK – MF DEK-MF decalcifying solution 111300410 Universal

  1. Decalcifying solutions

Products Size Code Description

Bouin 500ml 11400500 Picric acid/formalin

fixative 1000ml 114001000

2500ml 114002500

Saccomano 25ml 199001025 Fixative for cytology

speciments 1000ml 199001000

  1. Fixatives for histology

 

 

63

Products Size Code Description

Formaline 38-40% 1L 10561000

5L 10561000

Formaline 30% w/v 5L 10561000

1L 10561000

Formalin 10% solution

pH7.2-7.4 buffered

1L 10571000

5L 105755000

  1. Formalins

Products Size Code Description

Bio Clear 1L 10271000

5L 10275000

Prohyol 95 1L 10075000

5L 10071000

Prohyol absolute 1L 10775000

5L 10771000

  1. Solvents

 

 

64

Products Size Code Description

Paraffin wax histology 56-58°C 1kg 313110

Lepka 100ml 059801

Eurokit 100ml 0405003

Canada balsam 100ml 157160598

  1. Mounting and embed media

 

 

65

  • Acute toxicity (oral, dermal, inhalation), category 4
  • Skin irritation, categories 2, 3
  • Eye irritation, category 2A
  • Skin sensitization, category 1
  • Specific target organ toxicity following single exposure, category 3

o Respiratory tract irritation

o Narcotic effects

Acute toxicity (oral, dermal, inhalation), categories 1, 2, 3

  • Flammable gases, category 1
  • Flammable aerosols, categories 1, 2
  • Flammable liquids, categories 1, 2, 3
  • Flammable solids, categories 1, 2
  • Skin corrosion, categories 1A, 1B, 1C
  • Serious eye damage, category 1