We offer the best prices for these reagents made by our office in Bulgaria
contact for prices lieven@gentaur.com
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Hematoxylin stains
Products Code Size Description
Gill’s hematoxylin I 111601350 500ml For standard haematoxylin-eosin
staining of tissue sections. For nuclei. 111601310 1l
Gill’s hematoxylin II 111601450 500ml For standard haematoxylin-eosin
staining of tissue sections. For nuclei. 111601410 1l
Gill’s hematoxylin III 111601550 500ml For standard haematoxylin-eosin
staining of tissue sections. For nuclei 111601510 1l
Gill I Gill II Gill III
Mayer’s hematoxylin 111600250 500ml For standard haematoxylin-eosin
staining of tissue sections. For nuclei 111600210 1l
Harris’ hematoxylin 111600450 500ml For standard haematoxylin-eosin
staining of tissue sections. For nuclei 111600410 1l
Weigert’s Hematoxylin 130880100 100 test For trichrome stains
130880250 250ml
130880500 500ml
130880010 1l
Harris Mayer Weigert
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Eosin
Products Code Size Description
Eosin Y 1% aqueous sol. 111000050 50ml Standard haematoxylin-eosin staining
111000300 300ml for cytoplasm
111000250 500ml
111000210 1l
Eosin Y 5% aqueous sol. 111000050.5 50ml Standard haematoxylin-eosin staining
111000250.5 500ml for cytoplasm
111000210.5 1l
Eosin Y 1% aqueous sol. Eosin Y 5% aqueous sol.
Eosin Y 0.5% alcoholic sol. 111000305 50ml Standard haematoxylin-eosin staining
111000325 250ml for cytoplasm
111000350 500ml
111000310 1l
Eosin-Phloxin B alcoholic sol. 111002005 50ml Standard haematoxylin-eosin staining
111002025 250ml for cytoplasm
111002050 500ml
111002010 1l
Eosin Y 0,5% alcoholic sol. Eosin-Phloxin B alcoholic sol.
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Staining solutions for histology and cytology
Products Code Size Description
Alcian blue pH1 162802050 100test For demonstration of acid mucins in
tissue sections 162802250 250ml
162802500 500ml
162802100 1l
Alcian blue pH2.5 161802050 100test For demonstration of carboxylated and
some weakly sulfated mucins in tissue
sections
161802250 250ml
161802500 500ml
161802100 1l
Alcian blue pH1 – P.A.S. 111200100.1 100test For neutral and acid mucins in tissue
sections 111200101.1 100ml
111200250.1 250ml
111200500.1 500ml
111200010.1 1l
Alcian blue pH2.5 – P.A.S. 111200100 100test For neutral and acid mucins in tissue
sections 111200101 100ml
111200250 250ml
111200500 500ml
111200010 1l
Alcian Yellow – Toluidine Blue 111300100 100test For screening patient specimens for
Helicobacter pilory 111300250 250ml
Azan Trichrome 121111100 100test Method for connective tissue, especially
to show muscle fibers, collagen,
reticulum, kidney glomerular stroma in
tissue sections
121111250 250 ml
121111010 1l
Brilliant Cresyl Blue for
reticulocytes
111400050 100test Blood smears examination for
reticulocytes 111400125 125ml
111400250 250ml
111400500 500ml
111401000 1l
Bennhold’s Congo red for amyloid 131003050 100test Stain for amyloid deposits in tissues
131003250 250ml
131003500 500ml
131003100 1l
Highman’s Congo red for amyloid 131103205 100test Stain for amyloid deposits in tissues
131103250 250ml
131103500 500ml
131103100 1l
CytoMax – fast Papanicolau for
cyto PAP smear
221222050 100test For cyto PAP and other smears
(cytological specimens) 221222100 100ml
221222250 250ml
221222500 500ml
Giemsa stain 111120100 100test Giemsa concentrate solution
111120250 250ml
111120500 500ml
111120010 1l
Gomori silver impregnation 114080100 100test Method to show argyrophilic reticular
fibers in connective tissue
Gram for microbiology 100802100 100test Differentiation between Gram positive
and Gram negative bacteria in different
specimens
100802125 100ml
100802250 250ml
100802500 500ml
100802010 1l
Grocott’s methenamine silver
nitrate method for fungi
143823100 100test To identify fungi in tissue section
143823010 100ml
Haematocolor (hematocolor) 111316050 100test Blood smear examinations
111316100 100ml
111316250 250ml
111316500 500ml
Leishman stain 113273100 100test Blood smear examinations
113273125 125ml
113273250 250ml
113273500 500ml
Leishman-Wright stain 113273100W 100test Blood smear examinations
113273125W 125ml
113273250W 250ml
113273500W 500ml
Mallory trichrome 120802100 100test Staining solution for connective tissue
Masson trichrome Goldner 110802100.1 100test Staining solution for connective
tissue – collagen and muscle fiber
Masson trichrome Standard 110802100 100test Staining solution for connective
tissue – collagen and muscle fiber
May Grunwald 111200100 100test Fixative for MGG blood smear
examinations 111200250 250ml
111200500 500ml
111200010 1l
May Grunwald – Giemsa 118080100 100test Blood smear examination
118080010 1l
Methenamine Silver Impregnation
(P.A.S.M.)
114382100 100test Kit for histology (lamina basalae,
argyrophil cells etc.).
Methylene Blue Loffler 112000100 100test Alkaline stain for cell nuclei in smears
from different origin and pathogens 112000250 250ml
112000500 500ml
112000010 1l
New Methylene Blue for
reticulocytes
1114003050 100test Blood smear examination for
reticulocytes 1114003125 125ml
1114003250 250ml
Oil Red O for lipids in frozen
sections
122092100 100test Lipid staining in frozen sections
122092125 125ml
122092250 250ml
Orcein 155802100 100test To demonstrate elastic fibers in tissue
sections, recomended for vascular
pathology
155802250 250ml
Papanicolau – OG6 112220250 250ml Stain solution I for cyto PAP smears
112220500 500ml
112220010 1l
Papanicolau – EA65 112230250 250ml Stain solution II for cyto PAP smears
112230500 500ml
112230010 1l
Papanicolau – EA50 112230250.1 250ml Stain solution II for cyto PAP smears
112230500.1 500ml
11223010.1 1l
Periodic Acid Schiff (P.A.S.) 113080100 100test Examination of mucosubstances
(mucopolysaccharides), lamina
basalae, glycogen deposits in liver etc.
in tissue sections
113080101 100ml
113080250 250ml
113080500 500ml
13
Rapihem 111317050 100test Blood smears, other cytological
specimens and fine needle aspirations
examination
111317250 250ml
111317500 500ml
111317100 1l
Sudan III Propylene Glycol 112700050 100test Triglyceride staining in frozen sections
112700250 250ml
112700500 500ml
112700100 1l
Sudan III alcohol solution 112700050A 100test Staining of triglycerides in frozen tissue
sections. 112700250A 250ml
112700500A 500ml
112700100A 1l
Toluidine Blue stain for hystology 123001050 100test To demonstrate metachromatic
substances and mast cells in tissue
sections
123001250 250ml
123001500 500ml
123001100 1l
Van Gieson Trichrome stain 130802100 100test Staining for connective tissue
130802125 125ml
130802250 250ml
130802500 500ml
Weigert for elastic fibers 124001100 100test To demonstrate elastic fibers in tissue
sections 124001125 125ml
14
Wright’s Stain for cytology 113273100WP 100test Fine needle aspirations and other
cytological specimens examination 113273125WP 125ml
113273250WP 250ml
113273500WP 500ml
Ziehl Neelsen cold 116450050 100test Microscopic examination of
Mycobacteria 116450250 250ml
116450500 500ml
15
ALCIAN BLUE pH 1
number of tests 100
procedure time 50 min. (20 min. microwave oven modification)
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Weakly and strongly sulfated acid mucins Blue
Nuclei Red to pink
Other tissue elements Pale pink
Alcian blue is used for the demonstration of acid mucins, which can be produced by several types of epithelial and connective tissue tumors. Acid mucins also
play role in collagen diseases and early stages of atherosclerosis.
Reagents:
A – Alcian blue staining solution рН 1 B – Nuclear Fast Red staining solution
Alcian blue pH1 reaction – intestine
16
ALCIAN BLUE pH 2,5
number of tests 100
procedure time ~50 min. (20 min. microwave oven modification)
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, in glass dark
bottle, tightly closed
Carboxylated and some weakly sulfated
acid mucins
Blue
Nuclei Red to pink
Other tissue elements Pale pink
Alcian blue is used for the demonstration of acid mucins, which can be produced by several types of epithelial and connective tissue tumors. Acid mucins also
play role in collagen diseases and early stages of atherosclerosis.
Reagents:
A – Alcian blue staining solution рН 2,5 B – Nuclear Fast Red staining solution
Alcian blue pH2,5 reaction – intestine
17
ALCIAN BLUE pH 1 – P.A.S.
number of tests 100
procedure time ~50 min. (20 min. microwave oven modification)
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Weakly and strongly sulfated acid mucins Blue
Neutral mucins Magenta
Mixtures of acid and neutral mucins Bluish to reddish purple
Nuclei (if contra-stained) Blue to black
Alcian blue and PAS reactions can be combined to demonstrate both neutral
and acid mucins in tissue sections.
Reagents:
A – Alcian blue staining solution рН 1
C – Schiff Reagent
B – Periodic acid solution
Alcian blue pH1-PAS reaction – intestine
18
ALCIAN BLUE pH 2,5 – P.A.S.
number of tests 100
procedure time ~50 min. (20 min. microwave oven modification)
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, in glass dark
bottle, tightly closed
Carboxylated and some faintly acid
sulfomucins
Blue
Neutral mucins Magenta
Mixtures of acid and neutral mucins Bluish to reddish purple
Nuclei (if contra-stained) Blue to black
Alcian blue and PAS reactions can be combined to demonstrate both neutral
and acid mucins in tissue sections.
Reagents:
A – Alcian blue staining solution рН 2,5
C – Schiff Reagent
B – Periodic acid solution
Alcian blue pH2,5 reaction – intestine
19
ALCIAN YELLOW -TOLUIDINE BLUE
number of tests 100
procedure time ~25 min.
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Helicobacter pilory Dark blue
Mucins Yellow
Background Blue scale
Staining solution for screening patient specimens for Helicobacter pilory –
spiral shaped bacterium implicated in gastric inflammation, peptic ulcers and gastric
cancer.
Reagents:
А – Periodic acid
С – Alcian yellow staining solution
Е – Sodium hydroxide solution
В – Sodium bisulfite solution
D – Toluidine blue staining solution
Alcian yellow-Toluidine blue reaction – intestine
20
AZAN TRICHROME
number of tests 100
procedure time ~100 min.
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Collagen, reticulum, and kidney glomerular stroma Deep blue
Muscle Orange
Erythrocytes Red
Recommended method for connective tissue, especially to show muscle
fibers, collagen, reticulum, kidney glomerular stroma in tissue sections.
Reagents:
А – Azocarmine solution
C – Differentiation solution
E – Staining solution
В – Aniline solution
D – Phosphomolybdic acid solution
Azan trichrome staining: A) kidney; B)uterus
A B
21
BRILLIANT CRESYL BLUE
number of tests 100
procedure time ~35 min.
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Reticulocytes Greenish to blue with dark blue ribonucleoprotein granules inside
Erythrocytes Greenish to blue
Blood smears examination for reticulocytes. Brilliant cresyl blue is more used
in hematology laboratories than in histology laboratories. It is invaluable for the staining
of reticulocytes whereas its use in histology is limited to vital staining.
Reagents:
Brilliant Cresyl Blue physiological solution
Brilliant Cresyl Blue – blood smear
22
BENNHOLD’S CONGO RED
number of tests 100
procedure time ~90 min.
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Amyloid Pink to red (apple-green birefringence
with polarized light)
Nuclei Blue
Stain for amyloid deposits in tissues. Appropriate for tissue sections from
5-12μm. Amyloid deposits are formed in tissues spontaneously or in association with
a wide range of diseases. In cases of suspected amyloidosis, thick sections at 12 μm
may be beneficial in demonstrating minute deposits of amyloid.
Reagents:
A – Congo red staining solution according
Benhold
C – Mayer’s haematoxylin solution
B – Sodium hydroxide solution
Bennhold’s congo red for amyloid – kidney
23
HIGHMAN’S CONGO RED
number of tests 100
procedure time ~20 min.
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Amyloid Pink to red (apple-green birefringence
with polarized light)
Nuclei Blue if contra-stained
Stain for amyloid deposits in tissues. Suitable for paraffin sections fixed in
10% formalin. Amyloid deposits are formed in tissues spontaneously or in association
with a wide range of diseases. In cases of suspected amyloidosis, thick sections at 12
μm may be beneficial in demonstrating minute deposits of amyloid.
Reagents:
A- Congo red staining solution according
Highman
B- Potassium hydroxide solution
Highman’s congo red for amyloid – kindey
24
CYTOMAX
number of tests 100
procedure time 3 min.
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Nuclei Violet-blue to black
Cytoplasm (eosinophilic) Pink
Cytoplasm (basophilic) Turquoise to blue
Cytoplasm (metaplasia cells) With both blue and pink
Eritrocytes Red
Microorganisms Blue-violet
Stain solution for cyto PAP and other smears (cytological specimens).
Reagents:
А – Harris`s haematoxylin solution
C – Modified polychromic EA65 solution
B – 2-Propanol Absolute
D – 80% 2-Propanol
Cytomax – cyto PAP smear
25
GIEMSA STAIN
number of tests 100
procedure time ~40 min.
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Lymphocytes Light blue cytoplasm
Monocytes U shaped nucleus
Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (with dark violet to black granules)
Platelets Violet
Eritrocytes Light red
Blood parasites Red
Blood smears examination.
Reagents:
Giemsa concentrate solution
Giemsa stain – blood smear
26
GOMORI SILVER IMPREGNATION
Gomori silver impregnation – kidney
number of tests 100
procedure time ~30 min.
product validity 1 year (under recomended conditions)
storage conditions 15-25°C, protected from fire and sunlight, in glass dark bottle,
tightly closed
Reticular fibers and nervous cells Black
Connective tissue Tobacco brown
Collagen Gold yellow
Recommended method to show argyrophilic reticular fibers in connective tissue, especially to differentiate collagen fibers from connectivum.
Reagents:
А – Potassium permanganate solution
С – Oxalic acid solution
E – Ammoniacal silver solution
G – Sodium hyposulphite solution
В – Acid activating buffer
D – Ferric ammonium sulfate solution
F – Neutral formalin solution
27
GRAM
number of tests 100
procedure time 5 min.
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Gram positive microorganisms Blue
Gram negative microorganisms Red
Differentiation between Gram positive and Gram negative bacteria in different
specimens.
Reagents:
A – Crystal Violet solution
C – Decolorising solution
B – Lugol’s solution for Gram
D – Safranin O alcoholic solution
Gram staining – bacterial speciments
28
GROCOTT for fungi
Grocott’s method for fungi – gastric tissue
number of tests 100
procedure time ~100 min. (~25 min. – microwave oven modification)
product validity 1,5 years (under recomended conditions)
storage conditions 4°C, protected from fire and sunlight, tightly closed
Fungi Black
Mucin Grey
Background Green
To identify fungi in tissue sections. The mucopolysaccharide components of
the fungal cell wall are oxidized to release aldehyde groups. The aldehyde groups then
react with the silver nitrate, reducing it to a metallic silver, rendering them visible.
Reagents:
А – Chromic acid solution
С – Methenamine solution
E – Borax water solution
G – Sodium thiosulfate solution
В – Sodium hydrogensulfite solution
D – Silver nitrate solution
F – Gold chloride water solution
H – Light Green solution
29
HAEMATOCOLOR (HEMATOCOLOR)
number of tests 100
procedure time 1 min.
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Blood smears examination.
Reagents:
A – Methanol absolute
C – Haematocolor Solution II
B – Haematocolor Solution I
D – Haematocolor Phosphate buffer
Lymphocytes Bluish
Monocytes U shaped nucleus
Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (dark stained due to the large histamine
granules)
Platelets Purple
Eritrocytes Purple-pinkish
Haematocolor – blood smear
30
LEISHMAN STAIN
number of tests 100
procedure time 7 min.
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Blood smears examination.
B – Buffered water
Reagents:
A – Leishman staining solution
Leishman stain – blood smear
Lymphocytes Bluish
Monocytes U shaped nucleus
Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (dark stained due to the large histamine
granules)
Platelets Pink-violet
Eritrocytes Pink
31
LEISHMAN-WRIGHT STAIN
number of tests 100
procedure time 7 min.
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Blood smears examination.
B – Buffered water
Reagents:
A – Leishman-Wright staining solution
Leishman-Wright stain – blood smear
Lymphocytes Bluish
Monocytes U shaped nucleus
Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (dark stained due to the large histamine
granules)
Platelets Pink-violet
Eritrocytes Pink
32
MALLORY TRICHROME
number of tests 100
procedure time ~20 min.
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Nuclei Red
Erythrocytes Orange red
Muscle Red
Collagen Blue
Staining solution for connective tissue.
Reagents:
А – Acid fuchsin staining solution
C – Mallory’s staining solution
B – Phosphomolybdic acid solution
Mallory trichrome – placenta
33
MASSON TRICHROME STANDARD
number of tests 100
procedure time ~40 min (+/- pre-fixation time).
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Nuclei Black
Muscle fibers, cytoplasm, erythrocytes Red
Collagen, mucus Blue
Staining solution for histology. Collagen and muscle fiber stain differentially
when treated sequentially with Acid Fuchsin, PTA/PMA and Aniline Blue.
Reagents:
A- Weigert’s Haematoxylin solution A
C- Acid fuchsin staining solution
E- Phosphotungstic acid solution
G- Acetic acid solution
B- Weigert’s Haematoxylin solution B
D- Phosphomolybdic acid solution
F- Masson’s aniline blue solution
Masson trichrome standard – uterus
34
MASSON TRICHROME GOLDNER
Masson trichrome Goldner – uterus
number of tests 100
procedure time ~40 min. (+/- pre-fixation time).
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Staining solution for histology. Collagen and muscle fiber stain differentially
when treated sequentially with Acid Fuchsin, PTA and Light green.
Reagents:
A- Weigert’s Haematoxylin solution A
C- Acid fuchsin staining solution
E- Light green staining solution
Nuclei Black
Muscle fibers, cytoplasm, erythrocytes Red
Collagen
B- Weigert’s Haematoxylin solution B
D- Phosphotungstic acid solution
Greenish
35
MAY GRUNWALD STAIN
number of tests 100
procedure time ~2 min. (+ Giemsa co-staining)
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Supplementary stain for May Grunwald – Giemsa blood smear examination.
Reagents:
May – Grunwald Stain solution
White blood cells Faintly shaped
Erythrocytes Pinkish-red
May Grunwald stain – blood smear
May Grunwald stain only May Grunwald – Giemsa
36
MAY GRUNWALD – GIEMSA
number of tests 100
procedure time ~10-35 min.
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Blood smear examination and cytodiagnosis.
Reagents:
A – Methanol absolute
C – Giemsa staining solution
Lymphocytes Light blue cytoplasm
Monocytes U shaped nucleus
Granulocytes Neutrophils (3-6 lobed nucleus), eosinophils (2 lobed nucleus), basophils (with dark violet to black granules)
Platelets Violet
Eritrocytes Reddish brown
Blood parasites Red
B – May-Grunwald staining solution
D – Sorenson buffer pH7
May Grunwald Giemsa – blood smear
37
METHENAMINE SILVER IMPREGNATION – P.A.S.M.
P.A.S.M. – kidney
number of tests 100
procedure time ~2 h.
product validity 1 year (under recomended conditions)
storage conditions 4 °C, in dark glass bottle, protected from fire and sunlight,
tightly closed
Kit for histology (lamina basalae, argyrophil cells etc.).
Reagents:
А – Periodic acid solution
C – Hexamethylene tetraamine solution
E – Gold chloride solution
Lamina basalae Black
Nuclei and background Pinkish-red and green if contra-stained
Positive control Skin, kydney
В – Silver nitrate solution
D – Sodium tetraborate solution
F – Fixative
38
METHYLENE BLUE LOFFLER
Methylene Blue Loffler: A) mouth epithel; B)blood smear
number of tests 100
procedure time ~5 min.
product validity 2 years (under recomended conditions)
storage conditions 15-25 °C, protected from fire and sunlight, tightly closed
Alkaline stain for cell nuclei in smears from different origin. Pathogens (bacteria) examination.
Reagents:
Methylene Blue solution according Loffler
Bacteria Blue
Leucocytes nuclei Blue
Epithelial cells cytoplasm Slightly blue
Epithelial cells nuclei Blue
A B
39
NEW METHYLENE BLUE
New Methylene Blue – blood smear
number of tests 100
procedure time ~20 min.
product validity 2 years (under recomended conditions)
storage conditions 15-25 °C, protected from fire and sunlight, tightly closed
Blood smears examination for reticulocytes. Reticulocytes are juvenile red
cells, which contain remnants of basophilic ribonucleoproteins. This material reacts
with the dye to form a bluish black precipitate of granules or filaments.
Reagents:
New Methylene Blue water, isotonic solution, tilled with anticoagulant
Reticulocytes Greenish to blue with dark blue
ribonucleoprotein granules inside
Erythrocytes Greenish to blue
40
OIL RED O
Oil red O – omentum cryostate section
number of tests 100
procedure time ~40 min.
product validity 2 years (under recomended conditions)
storage conditions 15-25 °C, protected from fire and sunlight, tightly closed
Lipid staining in frozen sections.
Reagents:
A – Propylene glycol absolute
C – Propylene glycol 85% water solution
Nuclei Blue to black
Lipids
B – Staining solution Oil red O
D – Mayer’s haematoxylin solution
Red
41
ORCEIN for elastic fibers
number of tests 100
procedure time ~30 min
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Elastic fibers Purplish red-brown
Background Almost colourless
To demonstrate elastic fibers in tissue sections; recommended for vascular
pathology.
Reagents:
А – Potassium permanganate solution
С – Oxalic acid solution
Е – Orcein solution according Shikata
В – Acid activation buffer
D – Incubation media
F – Differentiation solution
Orcein for elastic fibers: A) blood vessels; B) kidney
A B
42
PAPANICOLAU
number of tests 100
procedure time ~25 min
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Stain solution for cyto PAP smears.
N.B! EA65 ( Biotech Ltd.) is an alternative for EA50 stain, providing more intensive cytoplasm staining!
Reagents:
А – Harris`s haematoxylin solution
C – EA50/65 polychromic stain solution
В – Orange G-6 stain solution
Papanicolau for cyto PAP smears: A) EA 50; B)EA 65
Nuclei Orange-brown to bluish-black
Cytoplasm (keratinized) Orange-pink
Cytoplasm (basophilic) Blue-green (EA50) to turquoise (EA65)
Cytoplasm (metaplasia cells) With both blue and pink
Cytoplasm (eosinophilic) Pink
Eritrocytes Red
Microorganisms Blue-violet to blue-black
A B
43
PERIODIC ACID SCHIFF (P.A.S.)
PAS reaction – gastric tissue (stomach)
number of tests 100
procedure time ~35 min.
product validity 2 years (under recomended conditions)
storage conditions 15-25 °C,in dark glass bottle, protected from fire and sunlight,
tightly closed
Examination of mucosubstances (mucopolysaccharides), lamina basalae,
glycogen deposits in liver etc. in tissue sections.
Reagents:
A – Periodic acid solution
C – Mayer’s haematoxylin solutionn
Glycogen, fungi, neutral mucosubstances, and some lamina basalae Magenta
Nuclei
B – Schiff Reagent
Blue
44
RAPIHEM
number of tests 100
procedure time ~ 40 sec.
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Cells Clear shaped morphology (nucleus,
cytoplasm), due to specimen origin! Enabling interpretation of their methabolitic
condition
Blood smears, other cytological specimens and fine needle aspirations examination.
Recommendable for fast blood smears examination!
Reagents:
A – Rapihem fixative
С – Rapihem solution II
В – Rapihem solution I
D – Rapihem washing solution –
concentrate
A
Rapihem : A) Fine needle aspiration; B) Blood smear
B
45
SUDAN III – alcohol solution
Sudan III alcohol solution – omentum cryostate section
number of tests 100
procedure time ~35 min.
product validity 2 years (under recomended conditions)
storage conditions 15-25 °C, protected from fire and sunlight, tightly closed
Staining of triglycerides in frozen tissue sections.
Reagents:
A – 50% Ethanol
C – Mayer’s haematoxylin solution
Nuclei Blue to black
Lipids
B – Staining solution Sudan III (alcoholic
Orange
solution)
46
SUDAN III – Propylene Glycol
Sudan III Propylene Glycol – omentum cryostate section
number of tests 100
procedure time ~25 min.
product validity 2 years (under recomended conditions)
storage conditions 15-25 °C, protected from fire and sunlight, tightly closed
Triglycerides staining in frozen sections.
Reagents:
A – Staining solution Sudan III
C – 50% Propylene glycol water solution
Nuclei Blue to black
Lipids
B – 85% Propylene glycol water solution
D – Mayer’s haematoxylin solution
Orange
47
TOLUIDINE BLUE
Toluidine Blue staining: A) skin; B) uterus
number of tests 100
procedure time ~6 min.
product validity 2 years (under recomended conditions)
storage conditions 15-25 °C, protected from fire and sunlight, tightly closed
- To demonstrate mucopolysaccharides and other metachromatic substances
in tissue sections. 2. To demonstrate mast cells (with histamine, heparin metachromatic
granules) in tissue sections.
Reagents:
Toluidine blue solution
Tissue structure Visible like in standard Haematoxylin/
Eosin histology staining, but in blue scale.
Mast cells Violet metachromatic staining
Positive control Skin
A B
48
VAN GIESON TRICHROME
Van Gieson trichrome staining: A) skin; B) placenta
number of tests 100
procedure time ~35 min.
product validity 2 years (under recomended conditions)
storage conditions 15-25 °C, protected from fire and sunlight, tightly closed
Staining of connective tissue.
Reagents:
A – Weigert’s Haematoxylin solution B
C – Van Gieson’s picrofuchsin
Nuclei Black
Collagen fibers Red
Muscle, cornified epithelium, erythrocytes
B – Weigert’s Haematoxylin solution B
Yellow
A B
49
WEIGERT for elastic fibers
Weigert for elastic fibers – kidney
number of tests 100
procedure time ~50 min.
product validity 2 years (under recomended conditions)
storage conditions 15-25 °C, protected from fire and sunlight, tightly closed
To demonstrate elastic fibers in tissue sections; recommended for vascular
pathology.
Reagents:
А – Periodic acid solution
С – Weigert’s resorcin fuchsin soution
Е – Carmalum according to Mayer
solution
Elastic fibers Purplish red-brown
Nuclei
В – Alcoholic reagent for incubation box
D – Acid differentiation buffer
Red
50
WRIGHT’S STAIN for cytology
number of tests 100
procedure time ~2 min
product validity 2 years (under recomended conditions)
storage conditions 15 – 25 °C, protected from fire and sunlight, tightly closed
Fine needle aspirations and other cytological specimens examination
Reagents:
A – Methanol absolute
С – Concentrated Phosphate buffer
рН 6,8
В – Wrght’s staining solution
Wright’s Stain for cytology – fine needle aspiration
Cells Clear shaped morphology (nucleus,
cytoplasm), due to specimen origin! Enabling interpretation of their methabolitic
condition
Erythocytes Pinkish-green
51
COLD ZIEHL-NEELSEN
Ziehl-Neelsen stain – sputum
number of tests 100
procedure time ~45 min.
product validity 2 years (under recomended conditions)
storage conditions 15-25 °C, protected from fire and sunlight, tightly closed
Microscopic examination of Mycobacteria.
Higher concentration of basic fuchsin and phenol in Kinyoun’s carbol fuchsin solution
helps penetration of Mycobacteria’s cell wall and removes the need for pre-heating
the slides.
Reagents:
А – Kinyoun’s carbol fuchsin solution
С – Methylene blue (water) staining solution
Tubercle bacilli Bright red
Background
В – Acid alcohol solution
Light blue
52
- Reagents I
Method Reagent Stability
ALCIAN BLUE pH 1- PAS A – Alcian blue staining solution рН 1 2
B – Periodic acid solution 2
C – Schiff Reagent 2
ALCIAN BLUE pH 2.5– PAS A – Alcian blue staining solution рН 2,5 2
B – Periodic acid solution 2
C – Schiff Reagent 2
ALCIAN BLUE pH 2.5 A – Alcian blue staining solution рН 2,5 2
B – Nuclear Fast Red staining solution 2
ALCIAN BLUE pH 1 A – Alcian blue staining solution рН 1 2
B – Nuclear Fast Red staining solution 2
ALCIAN YELLOW –
TOLUIDINE BLUE
А – Periodic acid solution 2
В – Sodium bisulfite solution 2
С – Alcian yellow staining solution 2
D – Toluidine blue staining solution 2
Е – Sodium hydroxide solution 2
AZAN TRICHROMЕ А – Azocarmine solution 2
В – Aniline solution 2
C – Differentiation solution 2
53
D – Phosphomolybdic acid solution 2
E – Staining solution 2
BRILLIANT CRESYL BLUE Brilliant Cresyl Blue physiological solution 2
BENNHOLD’S CONGO RED A – Congo red staining solution according
Benhold
2
B – Sodium hydroxide solution 2
C – Mayer’s haematoxylin 2
HIGHMAN’ S CONGO RED A- Congo red staining solution according
Highman
2
B- Potassium hydroxide solution 2
CYTOMAX А – Harris`s haematoxylin solution 2
B – 2-Propanol Absolute 2
C – Modified polychromic EA65 solution 2
D – 80% 2-Propanol 2
EOSIN –
ACQUEOUS SOLUTION 1%
Eosin Y 1% water solution 2
EOSIN –
ACQUEOUS SOLUTION 5%
Eosin Y 5% water solution 2
EOSIN –
ALCOHOLIC SOLUTION 0.5%
Eosin Y 0.5% alcoholic solution 2
EOSIN-PHLOXINE
ALCOHOLIC SOLUTION
А – Mayer’s haematoxylin 2
В – Scott’ s solution (water) 2
С – Eosin-Phloxine staining solution 2
GIEMSA STAIN Giemsa concentrate solution 2
GILL’S I HAEMATOXYLIN Gill’s 1 Haematoxylin solution 2
54
GILL’S II HAEMATOXYLIN Gill’s 2 Haematoxylin solution 2
GILL’S III HAEMATOXYLIN Gill’s 3 Haematoxylin solution 2
GOMORI SILVER
IMPREGNATION
А – Potassium permanganate solution 2
В – Acid activating buffer 2
С – Oxalic acid solution 2
D – Ferric ammonium sulfate solution (alum) 2
E – Ammoniacal silver solution 1
F – Neutral formalin solution 2
G – Sodium hyposulphite solution 2
GRAM STAIN A – Crystal Violet solution 2
B – Lugol’s solution for Gram 2
C – Decolorising solution 2
D – Safranin O alcoholic solution 2
GROCOTT’S А – Chromic acid solution 2
В – Sodium hydrogensulfite solution 2
С – Methenamine solution 2
D – Silver nitrate solution 2
E – Borax water solution 2
F – Gold chloride water solution 2
G – Sodium thiosulfate solution 2
H – Light Green solution 2
HAEMATOCOLOR A – Methanol absolute 2
B – Haematocolor solution I 2
55
C – Haematocolor solution II
2
D – Haematocolor Phosphate buffer
2
HARRIS’S HAEMATOXYLIN Harris’s haematoxylin solution
2
LEISHMAN STAIN A – Leishman Staining solution
2
B – Buffered water
2
LEISHMAN- WRIGHT STAIN A – Leishman-Wright staining solution
2
B – Buffered water
2
MALLORY TRICHROMЕ А – Acid fuchsin staining solution
2
B – Phosphomolybdic acid solution
2
C – Mallory’s staining solution
2
MASSON TRICHROME
GOLDNER
A- Weigert’s Haematoxylin solution A
2
B- Weigert’s Haematoxylin solution B
2
C- Acid fuchsin staining solution
2
D- Phosphotungstic acid solution
2
E- Light green staining solution
2
MASSON TRICHROME
STANDARD
A- Weigert’s Haematoxylin solution A
2
B- Weigert’s Haematoxylin solution B
2
C- Acid fuchsin staining solution
2
D- Phosphomolybdic acid solution
2
E- Phosphotungstic acid solution
2
F- Masson’s aniline blue solution
2
G- Acetic acid solution
2
MAY GRÜNWALD – GIEMSA A – Methanol absolute
2
56
B – May-Grunwald staining solution 2
C – Giemsa staining solution 2
D – Sorenson (Sorensen) buffer pH7 2
MAYER’S HAEMATOXYLIN Мayer’s Haematoxylin solution 2
MAY – GRUNWALD STAIN May – Grunwald Stain solution 2
METHENAMINE
SILVER IMPREGNATION –
P.A.S.M
А – Periodic acid solution 2
В – Silver nitrate solution 1
C – Hexamethylene tetraamine solution 1
D – Sodium tetraborate solution 2
E – Gold chloride solution 1
F – Fixative 2
METHYLENE BLUE LӦFFLER Methylene Blue solution according Loffler
NEW METHYLENE BLUE New Methylene Blue water, isotonic solution,
tilled with anticoagulant
OIL RED O A – Propylene glycol absolute
B – Staining solution Oil red O
C – Propylene glycol 85% water solution
D – Mayer’s haematoxylin solution
ORCEIN А – Potassium permanganate solution
В – Acid activation buffer
С – Oxalic acid solution
D – Incubation media
Е – Orcein solution according Shikata
F – Differentiation solution
57
PAPANICOLAU А – Harris`s haematoxylin solution
В – Orange G-6 stain solution
C – EA50 polychromic stain solution
C – EA65 polychromic stain solution
PERIODOC ACID SCHIFF
(PAS)
A – Periodic acid solution
B – Schiff Reagent
C – Mayer’s haematoxylin solution
RAPIHEM A – Rapihem fixative
В – Rapihem solution I
С – Rapihem solution II
D – Rapihem washing solution – concentrate
SUDAN III
PROPYLENE GLYCOL
A – Staining solution Sudan III
B – 85% Propylene glycol water solution
C – 50% Propylene glycol water solution
D – Mayer’s haematoxylin solution
SUDAN III
ALCOHOL SOLUTION
A – 50% Ethanol
B – Staining solution Sudan III (alcoholic
solution)
C – Mayer’s haematoxylin solution
TOLUIDINE BLUE STAIN Toluidine blue solution
VAN GIESON TRICHROME
STAIN
A – Weigert’s Haematoxylin solution A
B – Weigert’s Haematoxylin solution B
C – Van Gieson’s picrofuchsin.
WEIGERT for elastic fibers А – Periodic acid solution
58
В – Alcoholic reagent for incubation box
С – Weigert’s resorcin fuchsin
D – Acid differentiation buffer
Е – Carmalum according to Mayer
WEIGERT’S HAEMATOXYLIN A – Weigert’s Haematoxylin solution A
B – Weigert’s Haematoxylin solution B
WRIGHT’S STAIN for cytology A – Methanol absolute
В – Wrght’s staining solution
С – Concentrated Phosphate buffer рН 6,8
COLD ZIEHL-NEELSEN A – Kinyoun’s carbol-fuchsin solution
B – Acetic alcohol solution
C – Methylene Blue solution
59
- Reagents II
Products Code Size Description
Acid ethanol Shikata 155802125E 125ml Differentiating agent
155802250E 250ml
155802500E 500ml
Acid ethanol Weigert 153812125C 125ml Differentiating agent
153812250C 250ml
153812500C 500ml
153812100C 1l
Hidrocloric Acid (1M) 1090570125 125ml Differenting agent
1090570250 250ml
1090570500 500ml
1090570100 1l
Kovach’s indole reagent 119002125 125ml For identification of indole
119002250 250ml
119002500 500ml
119002100 1l
Orcein Acetic 1558011125 125ml For chromosomal staining
60
Oxalic Acid 1099650125 125ml Decolorant
1099650250 250ml
1099650500 500ml
1099650100 1l
Phosphate buffer pH 6.8 113273125 125ml
113273250 250ml
113273500 500ml
113273100 1l
Phosphate buffer pH 7.2 111316125D 125ml
111316250D 250ml
111316500D 500ml
111316100D 1l
Picric Acid aqueous sol. 1.2% 110901125 125ml For fixatives mixtures
110901250 250ml
110901500 500ml
110901100 1l
Picric Acid alcoholic sol. 110902125 125ml For erythrocytes in trichrome staining
110902250 250ml
110902500 500ml
110902100 1l
Picrofuchsin 1159270125 125ml For cytoplasm, muscle and collagen
61
Potassium Methabisulfite 0.5% 105057125 125ml Differentiating agent
105057250 250ml
105057500 500ml
105057100 1l
Sodium Thiosulphate 5% 109950125 125ml Decolorant after treatment with lugol
solution 109950250 250ml
109950500 500ml
109950100 1l
Sulphuric Acid 0.5% 109981125 125ml For oxidation with permanganate
109981250 250ml
109981500 500ml
62
Product Supplementary Code Description
DEK – HL DEK-HL decalcifying solution 111300910 For fibrillar and
lamellar bone
structure Acid solution for DEK-HL decalcifying solution 111300910.1
Ammonium solution for DEK-HL decalcifying solution 111300910.2
DEK – HN DEK-HN decalcifying solution 111300510 For bone tissue
Sodium solution for DEK-HN decalcifying solution 111300510.1
DEK – MF DEK-MF decalcifying solution 111300410 Universal
- Decalcifying solutions
Products Size Code Description
Bouin 500ml 11400500 Picric acid/formalin
fixative 1000ml 114001000
2500ml 114002500
Saccomano 25ml 199001025 Fixative for cytology
speciments 1000ml 199001000
- Fixatives for histology
63
Products Size Code Description
Formaline 38-40% 1L 10561000
5L 10561000
Formaline 30% w/v 5L 10561000
1L 10561000
Formalin 10% solution
pH7.2-7.4 buffered
1L 10571000
5L 105755000
- Formalins
Products Size Code Description
Bio Clear 1L 10271000
5L 10275000
Prohyol 95 1L 10075000
5L 10071000
Prohyol absolute 1L 10775000
5L 10771000
- Solvents
64
Products Size Code Description
Paraffin wax histology 56-58°C 1kg 313110
Lepka 100ml 059801
Eurokit 100ml 0405003
Canada balsam 100ml 157160598
- Mounting and embed media
65
- Acute toxicity (oral, dermal, inhalation), category 4
- Skin irritation, categories 2, 3
- Eye irritation, category 2A
- Skin sensitization, category 1
- Specific target organ toxicity following single exposure, category 3
o Respiratory tract irritation
o Narcotic effects
Acute toxicity (oral, dermal, inhalation), categories 1, 2, 3
- Flammable gases, category 1
- Flammable aerosols, categories 1, 2
- Flammable liquids, categories 1, 2, 3
- Flammable solids, categories 1, 2
- Skin corrosion, categories 1A, 1B, 1C
- Serious eye damage, category 1