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RNA research products> miRNA related products> ExoQuick Exosome Precipitation Solution

Exosome Concentrating Reagent
ExoQuick Exosome Precipitation Solution Series

System Biosciences, LLC
Exosome
It is a reagent that can easily concentrate high-purity and high-yield Exosomes from body fluid samples such as serum and ascites and cell culture solutions. It has a lot of usage records.

There are ExoQuick for serum, plasma and ascites samples, ExoQuick-TC for urine, cerebrospinal fluid and cell culture samples, and GMP grade ExoQuick-CG.

* This product is for research use. It cannot be used for anything other than research.

Exosome isolatioBibliographypricerelated productsCheck it out!Recommended product articlesn with Exo Quick

What is an exosome?
ExoQuick-TC operation
ExoQuick FAQ
What is an exosome?

Exosomes are 30-200 nm membrane vesicles secreted by cells in vivo and cultured cells, and are present in body fluids such as blood, urine, amniotic fluid, and ascites, and in cell culture fluid. The miRNAs and proteins contained in exosomes differ depending on the type of cells that secrete and the physiological conditions. Therefore, analysis of miRNAs and proteins in exosomes is useful for searching for new biomarkers involved in various diseases. See the ExoCarta database for more information on miRNAs and proteins in exosomes .

FBS Exo-FBS Exosome-Depleted FBS with bovine exosomes removed
Electron micrograph of exosomes isolated from serum using ExoQuick (#EXOQ5A-1)

Features
Exosomes can be isolated more easily and efficiently than conventional methods using ultracentrifugation, antibody-bound magnetic beads, and chromatograms.
MiRNAs purified from exosomes concentrated with this product are useful for accurate miRNA profiling with the MicroRNA qPCR Array with QuantiMir and the SeraMir Exosome RNA Amplification Kit. In addition, the extracted protein is useful for analysis by electrophoresis.
It has been used in many papers.

Product lineup
ExoQuick ExoQuick-TC
ExoQuick-CG ExoQuick Plasma
Prep Kit ExoQuick-LP ExoQuick ULTRA ExoQuick-TC ULTRA
Features Standard Exo Quick-CG is GMP grade Fibrin can be reduced Lipoprotein can be reduced Albumin and Ig can be reduced Albumin and Ig can be reduced
serum ○ — — ○ ○ —
plasma — — ○ ○ ○ —
Ascites ○ — — — — —
urine — ○ — — — —
Cerebrospinal fluid — ○ — — — —
Cell culture medium — ○ — — — ○
* ExoQuick ULTRA series is recommended for collecting exosomes with higher purity .

Outline of operation method
Centrifuge the sample at 3,000 xg for 15 minutes and remove cells and cell debris.
Transfer the supernatant to a sterile tube and add ExoQuick or ExoQuick-TC (see table).
Let stand at 4 ° C (see table).
Centrifugation at 1,500 xg for 30 minutes causes exosomes to precipitate as pale yellow to white pellets. Remove the supernatant by suction.
Centrifuge again at 1,500 xg for 5 minutes, and then remove the remaining supernatant by suction.
Add sterile distilled water to dissolve the exosomes.

ExoQuick-TC operation video

Addition amount of each product
Sample type Product Sample amount ExoQuick (-TC, -CG) addition amount Standing time
Ascites ExoQuick 250 μl 63 μl One night
Serum / plasma 250 μl 63 μl in 30 minutes
urine ExoQuick-TC (-CG) 5 ml 1 ml
(1.6 ml) 6 hours to overnight
(12 hours to overnight)
Cerebrospinal fluid 5 ml 1 ml
(1.6 ml) 6 hours to overnight
(12 hours to overnight)
Cell culture medium 5 ml 1 ml
(1.6 ml) 6 hours to overnight
(12 hours to overnight)
Example of use

■ Yield comparison with other companies’ products

Exosome (Exosome, Exosome) Concentration Reagent ExoQuick Exosome Precipitation Solution
Exosomes were recovered from human serum or cell culture medium using ExoQuick / ExoQuick-TC and company A products, and the number of exosomes was measured using the CD63 ELISA Kit (# EXOEL-CD63A-1). It can be seen that ExoQuick / ExoQuick-TC has a higher yield of exosomes.
Upper figure Sample: Human serum (500 μl)
Lower figure: PC-3 Cell culture medium in which prostate cancer cells were cultured for 7 days ( using DMEM medium containing exosome- removed FBS ( Exo-FBS ))

■ Examples of exosome recovery from ascites

Exosome (Exosome, Exosome) Concentration Reagent ExoQuick Exosome Precipitation Solution
■ Examples of exosome recovery from ascites

Exosome (Exosome, Exosome) Concentration Reagent ExoQuick Exosome Precipitation Solution ExoQuick Exosome Precipitation Solution