Lipid Peroxidation

Lipideperoxidatie is een bekend voorbeeld van

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Lipideperoxidatie (MDA + HNE) Assay Kit

Lipideperoxidatie is een bekend voorbeeld van oxidatieve schade in celmembranen, lipoproteïnen en andere lipide-bevattende structuren. Peroxidatieve modificatie van onverzadigde fosfolipiden, glycolipiden en cholesterol kan in verschillende reacties optreden. Ze kunnen worden geactiveerd door i) vrije radicalen zoals oxylradicalen, peroxylradicalen en hydroxylradicalen afgeleid van ijzer-gemedieerde reductie van waterstofperoxide of ii) niet-radicalen zoals singlet zuurstof, ozon en peroxynitriet gegenereerd door de reactie van superoxide met stikstofmonoxide.

Malondialdehyde (MDA) en 4-hydroxyalkenalen zijn belangrijke giftige bijproducten van lipideperoxidatie. De meting van de hoeveelheden van dergelijke aldehyden komt dus overeen met een index van lipideperoxidatie  in vitro  en  in vivo . 4-Hydroxynonenal (4-HNE) is een belangrijk product van de peroxidatieve afbraak van ω-6 meervoudig onverzadigde vetzuren (PUFA). Het bezit cytotoxische, hepatotoxische, mutagene en genotoxische eigenschappen. Verder werden verhoogde niveaus van HNE gevonden in plasma en verschillende organen onder omstandigheden van oxidatieve stress. In feite is MDA in veel gevallen het meest voorkomende individuele aldehyde als gevolg van lipideperoxidatie. In vitro  kan MDA eiwitten, DNA, RNA en vele andere biomoleculen veranderen.

 

Lipid Peroxidation biovision
Lipid Peroxidation biovision

De LPO- assaykit van Bioquochem   meet MDA- en HNE-concentraties als een index van lipideperoxidatie. Ten eerste initieert zuur-gekatalyseerde aanval op de 3-positie van de indolring de reacties tussen indolen en aldehyden (MDA en HNE). Als resultaat geeft deze reactie een diindolylalkaan (chromofoor) met een maximale absorptie in het gebied van 580-620 nm.

In onze test reageert een indol (reagens A) snel met MDA en HNE in zuur medium, wat een chromofoor (C) oplevert met een hoge molaire extinctiecoëfficiënt bij zijn maximale absorptiegolflengte van 586 nm.

 

  • Productoverzicht

    Lipideperoxidatie (MDA) Assay Kit (colorimetrisch/fluorometrisch) (ab118970) biedt een handig hulpmiddel voor gevoelige detectie van malondialdehyde (MDA).

    In het lipideperoxidatietestprotocol reageert het MDA in het monster met thiobarbituurzuur (TBA) om een ​​MDA-TBA-adduct te genereren. Het MDA-TBA-adduct kan gemakkelijk colorimetrisch (OD = 532 nm) of fluorometrisch (Ex/Em = 532/553 nm) worden gekwantificeerd. Deze test detecteert MDA-niveaus zo laag als 1 nmol/well colorimetrisch en 0,1 nmol/well fluorometrisch.

    De MDA-assay wordt ook wel een TBARS-assay genoemd.

    Samenvatting van protocol voor lipideperoxidatie-assay:
    – voeg TBA-oplossing toe aan monsters en standaarden, incubeer gedurende 60 minuten bij 95ºC, koel gedurende 10 minuten in ijsbad
    – breng over naar putjes van de microtiterplaat
    – analyseer met microtiterplaatlezer
    Voor hogere gevoeligheid, precipiteer met n-butanol, centrifugeer, droog en resuspendeer de pellet vóór analyse.

    lipid-peroxidation-assay-kit
    lipid-peroxidation-assay-kit

    Chinees protocol beschikbaar.  Zie protocollen hieronder.

    Voor een alternatieve MDA-assay, zonder de verwarmingsstappen die vereist zijn in de TBARS-assay, probeert u MDA-assay ab233471.

  • Opmerkingen:

    Lipideperoxidatie verwijst naar de oxidatieve afbraak van lipiden. In dit proces nemen vrije radicalen elektronen uit de lipiden (meestal in celmembranen), wat leidt tot celbeschadiging. Kwantificering van lipideperoxidatie is essentieel om oxidatieve stress te beoordelen. Lipideperoxidatie vormt reactieve aldehyden zoals malondialdehyde (MDA) en 4-hydroxynonenal (4-HNE) als natuurlijke bijproducten. Malondialdehyde (MDA) en 4-hydroxynonenal (4-HNE) worden vaak gebruikt als markers van lipideperoxidatie en om te testen op oxidatieve schade/oxidatieve stress.

    Gerelateerde producten

    Bekijk de oxidatieve stress marker en testgids, of de volledige metabolismetestgids voor meer informatie over metabolieten, metabolische enzymen, mitochondriale functie en oxidatieve stress, en ook hoe u de metabolische functie in levende cellen kunt testen met behulp van uw plaatlezer.

    Zie ook de populaire 4-HNE Assay Kit ab238538 als een alternatieve marker voor lipideperoxidatie en oxidatieve stress.


    Hoe andere onderzoekers Lipid Peroxidation Assay Kit ab118970 hebben gebruikt

    De MDA/TBARs-assaykit is gebruikt in publicaties in verschillende soorten monsters, waaronder:
    – Mens: serum 1 , extracten van primaire cellen van de hippocampus 2 , gekweekte A375-cellysaten 3 , plasma- en bloedplaatjesmonsters 4
    – Muis: neuronale cellysaten 5 , hartweefselextract 6 , plasma 7 , celextracten 8
    – Rat: hippocampusweefselextracten 9 , cardiomyocytenextracten van gekweekte cellen 10 , longlysaten 11
    – Varken: serum 12

    Referenties: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018, 4 – Mustafa AG et al. 2018, 5 – Murphy K et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE en Kang KS 2019

  • Platform

    Microplaatlezer

 

Lipid Peroxidation (MDA) Assay Kit
abx096010-100Assays 100 Assays
EUR 378
  • Shipped within 5-10 working days.

Lipid Peroxidation Colorimetric Assay Kit
K454-100
EUR 544

U-74389G (Lipid peroxidation blocker)
SIH-205-100MG 100 mg
EUR 174
  • U-74389G is an antioxidant which prevents iron-dependent lipid peroxidation. It prevents organ damage in intestinal cold storage, preservation and reperfusion injury (1-4).
Description: The substance U-74389G is a lipid peroxidation blocker. It is synthetically produced and has a purity of >99%. The pure substance is white solid which is soluble in 25 mg/ml DMSO, and in 20 mg/ml in ethanol; Insoluble in water.

U-74389G (Lipid peroxidation blocker)
SIH-205-500MG 500 mg
EUR 582
  • U-74389G is an antioxidant which prevents iron-dependent lipid peroxidation. It prevents organ damage in intestinal cold storage, preservation and reperfusion injury (1-4).
Description: The substance U-74389G is a lipid peroxidation blocker. It is synthetically produced and has a purity of >99%. The pure substance is white solid which is soluble in 25 mg/ml DMSO, and in 20 mg/ml in ethanol; Insoluble in water.

Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit
K2167-100 100 assays
EUR 586

Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit
K739-100
EUR 582

Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit
22906 200 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit
15991 200 tests
EUR 393
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Myeloperoxidase (MPO) Peroxidation Activity Assay Kit
K747-100
EUR 327

OxiSelect Myeloperoxidase Peroxidation Activity Assay Kit (Fluorometric)
STA-805 192 assays
EUR 577
Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase peroxidation activity within a sample.

Lipid A Lipopolysaccharide (LPS Lipid A / Endotoxin) Antibody
abx415762-1ml 1 ml
EUR 606
  • Shipped within 1 week.

Lipid A Antibody
abx023083-1ml 1 ml
EUR 739
  • Shipped within 5-10 working days.

Lipid Extraction & Polar/Neutral Lipid Separation Combo Kit (Chloroform Free)
MET-5009-C 50 preps
EUR 659
Description: This kit combines a chloroform free organic lipid extraction with further separation of polar and neutral lipids. The lipid extraction first extracts lipids from a crude lipid source to a chloroform free upper organic phase using a proprietary alcohol. After addition of a proprietary organic compound, the mixture is centrifuged to separate the phases. The recovered organic phase containing lipids is dried and then polar and neutral lipids are separated by adding a proprietary secondary alcohol and organic compound. The layers are separated, dried, and resuspended for further analysis.

Ceraplex Lipid Concentrate(1000X)
C3316-010 100ml
EUR 120

Ceraplex Lipid Concentrate(1000X)
C3316-050 500ml
EUR 246

Lipid Droplet Isolation Kit
MET-5011 50 preps
EUR 415
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

Lipid Quantitation Kit (Colorimetric)
STA-613 100 assays
EUR 531
Description: Cell Biolabs? Lipid Quantification Kit measures the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method, resulting in a simple colorimetric readout amenable to multi-well plate detection.

Lipid Quantification Kit (Fluorometric)
STA-617 100 assays
EUR 531
Description: The Lipid Quantification Kit (Fluorometric) uses a fluorometric detection system to quantify neutral lipids in a convenient 96-well microplate reader. The kit includes sufficient reagents for 100 assays.

Monophosphoryl Lipid A (Synthetic)
VAdv-Ly0012-1mg 1 mg
EUR 861
Description: Synthetic Monophosphoryl Lipid A, pure compound, a TLR4-based vaccine adjuvant.

Monophosphoryl Lipid A (Synthetic)
VAdv-Ly0012-5mg 5 mg
EUR 2511
Description: Synthetic Monophosphoryl Lipid A, pure compound, a TLR4-based vaccine adjuvant.

Synthetic Monophosphoryl Lipid A
VAdv-Ly0031 1 mg
EUR 2131
Description: Synthetic monophosphoryl lipid A, a TLR4 agonist vaccine adjuvant.

Anti-Lipid A antibody
STJ16100142 1 mL
EUR 289

Goat Lipid peroxide ELISA kit
E06L0263-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Lipid peroxide ELISA kit
E06L0263-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Lipid peroxide ELISA kit
E06L0263-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Lipid peroxide ELISA kit
E02L0263-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Lipid peroxide ELISA kit
E02L0263-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Lipid peroxide ELISA kit
E02L0263-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Lipid peroxide ELISA kit
E03L0263-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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