Separating and Passaging Cells in Tissue Culture

Splitting and Passaging Cells in Tissue Culture 1. Media warm and Trypsin-EDTA in a water bath (Trypsin-EDTA are made by diluting the stock by adding PBS 1/10 only) 1. cell divisions when theyre about 80-90% confluent 2. wash the cells with ~ 7ml PBS-EDTA (1 mM EDTA) 3. The aspiration of PBS-EDTA 4. + 1mL …

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